Circuli increments fish4/1/2023 ![]() A broken-line regression analysis on weight gain showed that the optimum dietary arginine level is 2.37% of diet (4.74% of dietary protein)Ĭhanges in digestive enzyme activity and histology were studied in Atractosteus tropicus embryos, larvae and juvenile periods. Also, significantly higher total nitric oxide synthase activity was recorded at 3.08% arginine level in comparison to 1.42% arginine. Significant improvements in lysozyme and myeloperoxidase activities and total immunoglobulin level were obtained by dietary arginine increment. ![]() Plasma total protein level significantly was increased in fish fed 2.54–3.08% arginine, and alanine aminotransferase activity and glucose level were significantly decreased in fish fed 2.22–2.54% and ≥1.88% arginine, respectively, compared to the group fed 1.42% arginine. ![]() Significant reductions in whole-body and muscle lipid contents were found by increment of arginine level and whole-body protein increased significantly in fish fed 2.22–2.54% arginine compared to those fed 1.42% arginine. Significant improvement in protein productive value was found at dietary arginine level of 2.54% compared to the fish fed 1.42% arginine. At the end of the feeding trial, fish fed ≥2.22% arginine showed significantly (P b 0.05) higher growth than those fed 1.42% arginine. Six isonitrogenous and isocaloric diets (50% crude protein and 17.7 kJ g−1 gross energy) were formulated to contain graded levels of arginine including 1.42, 1.88, 2.22, 2.54, 3.08 and 3.43% of diet (2.84–6.86% of dietary protein), and fed triplicate groups of fish (13.3 ± 0.2 g) to apparent satiation twice daily. undecimalis is very similar to other strictly marine carnivorous fish, and we suggest that weaning process should be started at 34 dah.Ī 9-week feeding trial was carried out to evaluate dietary arginine requirement of juvenile red sea bream. The acid protease zymogram showed two bands (RF = 0.32 and 0.51, respectively) at 34 dah. The alkaline protease zymogram showed two active bands, the first (26.3 kDa) at 25 dah onwards, and the second (51.6 kDa) at 36 dah. However, the maximum values for alkaline protease (6,500 U mg protein−1), trypsin (0.053 mU × 10−3 mg protein−1), and Leucine aminopeptidase (1.4 × 10−3 mU mg protein−1) were detected at 12 dah for chymotrypsin at 25 dah (3.8 × 10−3 mU mg protein−1), for carboxypeptidase A (280 mU mg protein−1) and lipase at 36 dah (480 U mg protein−1), for α-amylase at 7 dah (1.5 U mg protein−1), for acid phosphatases at 34 dah (5.5 U mg protein−1), and finally for alkaline phosphatase at 25 dah (70 U mg protein−1). During larviculture, all digestive enzymatic activities were detected with low values since yolk absorption, 2 days after hatching (dah) onwards. In this sense, our objective was to determine the changes of digestive enzymes activities using biochemical and electrophoretic techniques during 36 days of Common snook larviculture fed with live preys (microalgae, rotifers, and Artemia). However, larviculture remains as the main bottleneck for massive production. urophthamus is a precocious fish with a great capacity to digest all kinds of food items, including artificial diets provided from 13 dah.Ĭommon snook (Centropomus undecimalis) is one of the most important marine species under commercial exploitation in the Gulf of Mexico for this reason, interest in developing its culture is a priority. Three alkaline proteases isoforms (24.0, 24.8, 84.5 kDa) were detected at 8 dah using SDS–PAGE zymogram, corresponding to trypsin, chymotrypsin and probably leucine aminopeptidase enzymes, and only one isoform was detected (relative electromobility, Rf = 0.54) for acid proteases (pepsin-like) from 3 dah onwards using PAGE zymogram. Most digestive enzymes were present from yolk absorption (5–6 dah), except for the specific acid proteases activity (pepsin-like), which increase rapidly from 8 dah up to 20 dah. Several samples were collected including yolk-sac larvae (considered as day 1 after hatching) and specimens up to 60 dah. From yolk absorption (6 days after hatching: dah), larvae were fed Artemia nauplii until 15 dah, afterward they were fed with commercial microparticulated trout food (45% protein and 16% lipids) from 16 to 60 dah. The development of digestive enzymes during the early ontogeny of the Mayan cichlid (Cichlasoma urophthalmus) was studied using biochemical and electrophoretic techniques.
0 Comments
Leave a Reply.AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |